Sample Quantitation and Qualitation

Molecular Biology Core Facility Sample Quantitation and Qualitation Services

If you need to book instrument use, training, or consulation please email: Mbcf@etsu.edu

Sample quantity and quality are the greatest determinants of a successful molecular experiment. Your core houses instrumentation for several complementary methods to ensure that you are using the best samples for your downstream applications.

  • NanoDrop One

    NanoDrop OneThe NanoDrop One spectrophotometer accurately quantifies DNA, RNA, or protein from as little as one microliter of sample. Visualization of the absorbance spectra and onboard Acclaro Sample Intelligence technology makes it easy to identify potential contaminants. 

    The NanoDrop One can also 

    • Measure microarray or labeled proteins- quantify nucleic acids or proteins labeled with up to two fluorescent dyes (detection of dye concentration as low as 0.2 pM/mL)
    • Protein quantitation by A280, A205, BCA, Bradford, Lowry, or Pierce 660
    • Monitor growth rate of microbial cell cultures by OD600
    • Use custom methods for anything spectrally compatible
  • Qubit Fluorometer

    The Qubit Fluorometer is our most specific quantification instrument. 

    Use the Qubit for 

    • dsDNA, ssDNA, and RNA quantitation in 2 minutes
    • Protein quantitation in 15 minutes
    • MyQubit assays for anything spectrally compatible, including: mmmmm m   microRNA, c, cholesterol, ggggggggggalactose, glucose, glutamic acid, peroxide, and sucrose
  • Agilent 2100 Bioanalyzer and 2200 Tapestation


    Agilent BioanalyzerThe Agilent™ 2100 Bioanalyzer and  the Agilent™ 2200 Tapestation are  high-throughput microfluidics-based platforms for rapid analysis of DNA, RNA, and Protein.

    The Bioanalyzer RNA kits allow dependable and precise integrity checks and sample quantitation prior to any RNA-dependent application. This ensures the data from downstream research processes, such as gene expression microarray, qPCR, and transcriptome sequencing, are valid and reliable.

    Besides sample quality control of next-generation sequencing (NGS) libraries, additional research applications for the Bioanalyzer DNA assays include PCR amplicon analysis, multiplex PCR amplicons, gene expression analysis by RT-PCR, restriction fragment analysis, and the detection of targeted cleavage in gene editing studies.

Publications

Phillips Campbell, R.B., Duffourc, M.M., Schoborg, R.V., Xu, Y., Liu, X., KenKnight, B.H., Beaumont, E. “Aberrant fecal flora observed in guinea pigs with pressure overload is mitigated in animals receiving vagus nerve stimulation therapy.” American Journal of Physiology- Gastrointestinal and Liver Physiology. Oct 16, 311(4): G754-62. PMID: 27562060.

Chandley MJ, Crawford JD, Szebeni A, Szebeni K, and Ordway GA. "NTRK2 expression levels are reduced in laser captured pyramidal neurons from the anterior cingulate cortex in males with autism spectrum disorder." Molecular Autism volume 6, Article number: 28 (2015)

Szebeni A, Szebeni K, DiPeri T, Chandley MJ, Crawford JD, Stockmeier CA, and Ordway GA. "Shortened telomere length in white matter oligodendrocytes in major depression: potential role of oxidative stress." International Journal of Neuropsychopharmacology (2014), 17, 1579–1589.

Chandley MJ, Szebeni A, Szebeni K, Crawford J, Stockmeier CA, Turecki G, Kostrzewa RM, and Ordway GA. "Elevated gene expression of glutamate receptors in noradrenergic neurons from the locus coeruleus in major depression." International Journal of Neuropsychopharmacology, Volume 17, Issue 10, October 2014, Pages 1569–1578.

Chandley MJ, Szebeni K, Szebeni A, Crawford J, Stockmeier CA, Turecki G, Miguel-Hildalgo JJ, and Ordway GA. "Gene expression deficits in pontine locus coeruleus astrocytes in men with major depressive disorder." J Psychiatry Neurosci. 2013 Jul; 38(4): 276–284. PMID: 23415275

Dill BD, Dessus-Babus S, and Raulston JE. "Identification of iron-responsive proteins expressed by Chlamydia trachomatis reticulate bodies during intracellular growth." Microbiology (Reading). 2009 Jan;155(Pt 1):210-219. PMID: 19118361

Dessus-Babus S, Moore CG, Whittimore JD, and Wyrick PB. "Comparison of Chlamydia trachomatis serovar L2 growth in polarized genital epithelial cells grown in three-dimensional culture with non-polarized cells." Microbes Infect. 2008 Apr;10(5):563-70. PMID: 18396437

Guseva NV, Dessus-Babus S, Moore CG, Whittimore JD, and Wyrick PB. "Differences in Chlamydia trachomatis serovar E growth rate in polarized endometrial and endocervical epithelial cells grown in three-dimensional culture." Infect Immun. 2007 Feb;75(2):553-64. PMID: 17088348

Guseva NV, Dessus-Babus S,  Whittimore JD, Moore CG, and Wyrick PB. "Characterization of estrogen-responsive epithelial cell lines and their infectivity by genital Chlamydia trachomatis." Microbes Infect. 2005 Dec;7(15):1469-81. PMID: 16046168